هپس HEPES H4034 SIGMA
هپس HEPES H4034 SIGMA
هپس HEPES H4034 SIGMA
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H4034
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HEPES
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BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein/REAXYS Number:
883043
EC Number:
MDL number:
PubChem Substance ID:
Properties
grade
BioPerformance Certified
Quality Level
assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
cell culture | mammalian: suitable
competitive inhibition ELISA: suitable
impurities
endotoxin and total aerobic microbial count, tested
color
white
useful pH range
6.8-8.2
pKa (25 °C)
7.5
cation traces
Fe: ≤5 ppm
absorption
≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M
suitability
suitable for Western blot
suitable for component for culture media
application(s)
clinical research
diagnostic assay manufacturing
general analytical
life science and biopharma
foreign activity
DNase, RNase, NICKase, protease, none detected
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
Description
General description
HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.[1]
HEPES has been described as one of the best all-purpose buffers available for biological research.[2] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[3]
HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer. It demonstrates outstanding buffering capacity in the pH range of 6.8 to 8.2, boasting a pKa of 7.48 at 25°C. Widely acknowledged as one of the most versatile buffers, HEPES is extensively utilized in cell biology, biochemical, and biological research. A notable attribute is its non-reactivity with metal ions. Unlike many buffers, HEPES does not form significant complexes with most metals, making it suitable for applications involving metal ions without affecting their activity. This characteristic enhances its utility as a "non-coordinating buffer." In cell culture, HEPES excels in maintaining a stable physiological pH, even amidst fluctuations in carbon dioxide concentration resulting from cellular respiration. This sets it apart from bicarbonate buffers (NaHCO3), which, though commonly used, are less effective in pH stability.
HEPES also proves advantageous in various biological and biochemical processes. Its ampholytic nature makes it suitable as a separator for creating pH gradients in isoelectric focusing, a technique often employed in protein separation and analysis. Moreover, HEPES exhibits minimal interference with DNA-restriction enzyme reactions compared to buffers with fewer substituted amine groups, such as Tris. This makes it a preferred choice for applications involving DNA manipulation and analysis. Beyond these specific applications, HEPES is valuable in numerous other biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, coupled with exceptional pH buffering capacity and minimal interaction with other molecules, establishes HEPES as an indispensable tool across diverse research domains.
Application
HEPES has been used:
To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines[4][5]
As a component of platelet suspension buffer[6]
To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue[7]
As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay[8]
For the adjustment and maintenance of pH of biological solutions[8]
As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development[9]
For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells[10]
As a component of keratinocyte and fibroblast culture medium[11]
Features and Benefits
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
Negligible metal ion binding
Less toxic to cells than other buffers such as Tris and phosphate
Stable in a wide pH range
Tested for Endotoxins and Total Aerobic Microbial Count
Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease
Other Notes
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