کالای فیزیکی
تیازولیل بلوتترازولیم بروماید Thiazolyl Blue Tetrazolium Bromide M2128
تیازولیل بلوتترازولیم بروماید Thiazolyl Blue Tetrazolium Bromide M2128
تیازولیل بلوتترازولیم بروماید Thiazolyl Blue Tetrazolium Bromide M2128

تیازولیل بلوتترازولیم بروماید Thiazolyl Blue Tetrazolium Bromide M2128

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تیازولیل بلوتترازولیم بروماید M2128

MTT

Thiazolyl Blue Tetrazolium Bromide

لینک کالا در سایت سیکما ( کلیک کنید )

برگه انالیز کالا ( کلیک کنید )

دیتا شیت ( کلیک کنید )

M2128

Thiazolyl Blue Tetrazolium Bromide

98%

Synonym(s):
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide

Empirical Formula (Hill Notation):
C18H16BrN5S

CAS Number:
298-93-1
Molecular Weight:
414.32
Beilstein:
4081397
EC Number:
206-069-5
MDL number:
MFCD00011964
PubChem Substance ID:
24896603
NACRES:
NA.47

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems. Thiazolyl Blue Tetrazolium Bromide has been used for the following assays: